LOCUS       sequence-146501-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of GFP-tagged LC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Fung C, Lock R, Gao S, Salas E, Debnath J
  TITLE     Induction of autophagy during extracellular matrix detachment 
            promotes cell survival.
  JOURNAL   Mol Biol Cell. 2008 Mar . 19(3):797-806.
  PUBMED    18094039
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2008 Mar . 19(3):797-806."
COMMENT     Sequence Label: pBABEpuro GFP-LC3
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(296..718)
                     /codon_start=1
                     /gene="Map1lc3b"
                     /product="rat LC3 protein"
                     /label=LC3
                     /note="Atg8 family member found on autophagosomes"
                     /translation="PSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPV
                     LDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDE
                     DGFLYMVYASQETFGTALAVTYMSALKATATGREPCL"
     primer_bind     complement(779..800)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaccagggac atgacgacgt acacaaccca cacacggcag cagtggggac ttacacagtg
       61 taacataaca ttgggggtta gcagggacag ctgcatgcag ggagggtggg tgctgtgggg
      121 agccaggtcg agaggtggct gtgttggctt ccgtccgtga cagaggcagc tctcactgag
      181 attctcagac acgagtgagt gagtgaccgg ggatggtctg agtgtcacag tgggctccat
      241 gcaggtagca gggagcagag gcttgcttta gttggaagtg gctgtatgtc tgtcacaagc
      301 atggctctct tcctgttgct gttgccttca gagctgacat gtatgtaaca gccagtgctg
      361 tcccgaacgt ctcctgggag gcatagacca tgtacaggaa gccgtcttca tctctctcgc
      421 tctcgtacac ttcagagatg ggtgtggaca cactcaccat gctgtgccca ttcaccagga
      481 ggaagaaggc ttggttagca ttgagctgca ggcgccttct aattatcttg atgagttcgc
      541 tcatattcac gtgatcaggt acaaggaact tggtcttgtc caggacgggc agctgcttct
      601 cacccttgta tcgctctata atcactggga tcttggtggg gtgctgctcc cggatgagcc
      661 ggacatcttc cactctttgt tcgaagctcc ggcgctgttt gaaggtcttc tcggacggtc
      721 tagatctgag tccggacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
      781 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctt
//
