LOCUS       sequence-146504-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Destination vector - Position D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU1-D-lacZ
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     RBS             390..401
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(410..426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(410..426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(423..445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    434..450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    487..506
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      509..552
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(556..574)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        642..746
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcctgc agcgtctctc taaaacatga gaccgcatgc
       61 ggatccacta gtgcgaaaaa accccgccct gtcaggggcg gggttttttg cgttagttgt
      121 tagacagcgg cggcagcagc caagacagtt tacgttccgg tttggtcagc agacggatca
      181 gagaacgcag ctgctgggac ggacggtcgg tacgagcttc ttcagagtta cgccaagaag
      241 cgaacggcgg gtgagcagcc agacggttca gctgggtaac acccgggttt tcccagtcac
      301 gacgttgcag aacaacagcc agagagttac ccggagaaga agagatagcg tgcggaactt
      361 ccagcagcag tttcgggtca cccatgtcct ttctcctctt tgtaccatgg tcatagctgt
      421 ttcctgtgtg aaattgttat ccgctcacaa catatgtcta gaggtctcta tagcttctga
      481 gacgttctag aagcggccgc gaattccaga aatcatcctt agcgaaagct aaggattttt
      541 tttatctgaa attctgcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      601 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      661 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      721 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      781 c
//
