LOCUS       sequence-146505-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Destination vector - Position B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU1-B-RFP
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(61..88)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(104..175)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcctgc agcgtctctc cggaacatga gacctataaa
       61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca
      121 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
      181 tggctctagt agcgatctac actagcacta tcagcgttat taagcaccgg tggagtgacg
      241 accttcagca cgttcgtact gttcaacgat ggtgtagtct tcgttgtggg aggtgatgtc
      301 cagtttgatg tcggttttgt aagcacccgg cagctgaacc ggttttttag ccatgtaggt
      361 ggttttaact tcagcgtcgt agtgaccacc gtctttcagt ttcagacgca ttttgatttc
      421 acctttcaga gcaccgtctt ccgggtacat acgttcggtg gaagcttccc aacccatggt
      481 ttttttctgc ataaccggac cgtcggacgg gaagttggta ccacgcagtt taactttgta
      541 gatgaactca ccgtcttgca gggaggagtc ctgggtaacg gtaacaacac caccgtcttc
      601 gaagttcata acacgttccc atttgaaacc ttccgggaag gacagtttca ggtagtccgg
      661 gatgtcagcc gggtgtttaa cgtaagcttt ggaaccgtac tggaactgcg gggacaggat
      721 gtcccaagcg aacggcagcg gaccaccttt ggtaactttc agtttagcgg tctgg
//
