LOCUS       sequence-146506-        6141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_91_4xUAS_dCP
FEATURES             Location/Qualifiers
     source          1..6141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(22..813,814..882))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    987..1035
                     /note="synthetic polyadenylation signal"
     misc_feature    1049..1140
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1089..1108
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     protein_bind    1187..1211
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1316..1340)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1537..1546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1543..3195
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1609..1627)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3236..3357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3247..3266)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3301..3320
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3551..3908
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3551..3742
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3574..3594)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3759..3894
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3821..3840
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3939..4976
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             4955..4963
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    5000..5048
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5204..5221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5375..5963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5455..5474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat
       61 ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg
      121 tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc
      181 ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc
      241 tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc
      301 gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc
      361 gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc
      421 acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa
      481 gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat
      541 gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta
      601 gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca
      661 tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag
      721 gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc
      781 agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc
      841 aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata
      901 ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa
      961 ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg
     1021 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     1081 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     1141 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaaacaa gtttgtacaa
     1201 aaaagcaggc ttccgggagt actgtcctcc gagcggagta ctgtcctccg agcgggaagc
     1261 ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgaggaccca
     1321 gctttcttgt acaaagtggt atatcaagat ctgcgatcga gctcgcccgg ggatcgagcg
     1381 cagcggtata aaagggcgcg gggtggctga gagcatcagt tgtgaatgaa tgttcgagcc
     1441 gagcagacgt gccgctgcct tcgttaatat cctttgaata agccaacttt gaatcacaag
     1501 acgcatacca aacggcattc cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac
     1561 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg
     1621 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
     1681 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct
     1741 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
     1801 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
     1861 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg
     1921 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc
     1981 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
     2041 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc
     2101 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa
     2161 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt
     2221 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     2281 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     2341 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg
     2401 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta
     2461 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg
     2521 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca
     2581 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     2641 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa
     2701 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa
     2761 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     2821 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc
     2881 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac
     2941 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     3001 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     3061 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     3121 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     3181 aagatcgccg tgtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     3241 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     3301 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     3361 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     3421 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg
     3481 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     3541 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     3601 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3661 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3721 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3781 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3841 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3901 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gaagaagccc gaactcaccg
     3961 ctaccagcgt tgaaaaattt ctcatcgaga agttcgacag tgtgagcgac ctgatgcagt
     4021 tgtcggaggg cgaagagagc cgagccttca gcttcgatgt cggcggacgc ggctatgtac
     4081 tgcgggtgaa tagctgcgct gatggcttct acaaagaccg ctacgtgtac cgccacttcg
     4141 ccagcgctgc actacccatc cccgaagtgt tggacatcgg cgagttcagc gagagcctga
     4201 catactgcat cagtagacgc gcccaaggcg ttactctcca agacctcccc gaaacagagc
     4261 tgcctgctgt gttacagcct gtcgccgaag ctatggatgc tattgccgcc gccgacctca
     4321 gtcaaaccag cggcttcggc ccattcgggc cccaaggcat cggccagtac acaacctggc
     4381 gggatttcat ttgcgccatt gctgatcccc atgtctacca ctggcagacc gtgatggacg
     4441 acaccgtgtc cgccagcgta gctcaagccc tggacgaact gatgctgtgg gccgaagact
     4501 gtcccgaggt gcgccacctc gtccatgccg acttcggcag caacaacgtc ctgaccgaca
     4561 acggccgcat caccgccgta atcgactggt ccgaagctat gttcggggac agtcagtacg
     4621 aggtggccaa catcttcttc tggcggccct ggctggcttg catggagcag cagactcgct
     4681 acttcgagcg ccggcatccc gagctggccg gcagccctcg tctgcgagcc tacatgctgc
     4741 gcatcggcct ggatcagctc taccagagcc tcgtggacgg caacttcgac gatgctgcct
     4801 gggctcaagg ccgctgcgat gccatcgtcc gcagcggggc cggcaccgtc ggtcgcacac
     4861 aaatcgctcg ccggagcgca gccgtatgga ccgacggctg cgtcgaggtg ctggccgaca
     4921 gcggcaaccg ccggcccagt acacgaccgc gcgctaagga ggtaggtcga gtttaaactc
     4981 tagaaccggt catggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     5041 tttgtgtgtt cgaactagat gctgtcgacc gatgcccttg agagccttca acccagtcag
     5101 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     5161 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
     5221 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5281 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5341 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5401 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5461 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5521 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5581 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5641 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5701 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5761 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5821 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5881 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5941 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6001 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6061 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6121 cttggtctga cagcggccgc a
//
