LOCUS       sequence-146512-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses artificially dimerized Hook domain of Hook3 in bacteria 
            for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Schroeder CM, Vale RD
  TITLE     Assembly and activation of dynein-dynactin by the cargo adaptor 
            protein Hook3.
  JOURNAL   J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
  PUBMED    27482052
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-08-1- 1"; volume: "214"; 
            issue: "3"; pages: "309-18"
COMMENT     Sequence Label: pET28a-Hook3 aa 1-160-GCN4
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..54)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(667..687)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(734..754)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcttccttt cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag
       61 tgcggccgct tagaccagct tcttcaggcg ggcgacttca ttctctaagt gtgcgttctt
      121 ggagagcagt tcttcgactt tgtcttctaa ctgcttcacg ctgccgctac ctttactcat
      181 cagctcttga atggctgtca tgacaacatg ttgaacagat tcctccatca tcataatggc
      241 ttggatgtac tcttgcttct gttcacagtt cacagcacag cctaagatga gctgaagcat
      301 ccttccaagc tctgctgcat cagaatgctc cccaataagg ttcacatcag gaagggtaaa
      361 gtcattaatt tgctgtccta aaatctcatg attataatcc aagattcctt ttaaaatttt
      421 ctttaaattg cttatcttta gcctccaatt atctcctact tcagttttga ttctgtttag
      481 ccaattttca tcaaaatatg caggatctat cttttgaaga acctgggcca tcacaacccc
      541 attcgttaaa tcttccacgg tctggcatgg tgcatccaca ttaaatgtct ggatccaagt
      601 gaggaggctc tcgcacagct ccgcccgctc cagcgactct acgctgaaca tggtaccacc
      661 tccacccccc tgaaaataca gattttcgga tcctttgtag agctcatcca tgccatgtgt
      721 aatcccagca gcagttacaa actcaagaag gaccatgtgg tcacgctttt cgttgggatc
      781 tttcgaaagg acagattgtg tcgacaggta atggttgtct ggtaaaagga cagggccatc
      841 gccaattgga gtattttgtt gataatggtc tgctagttga acggaaccat cttcaacgtt
      901 gtggcgaatt ttgaagttag ctttgattcc at
//
