LOCUS       sequence-146514-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a triple mutant truncation of Hook3 in bacteria for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Schroeder CM, Vale RD
  TITLE     Assembly and activation of dynein-dynactin by the cargo adaptor 
            protein Hook3.
  JOURNAL   J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
  PUBMED    27482052
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-08-1- 1"; volume: "214"; 
            issue: "3"; pages: "309-18"
COMMENT     Sequence Label: pET28a-Hook3 Q147A, M151A, I154A
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(802..822)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(869..889)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcttat ggactgttag ggtcttctat agaatcagat tgattgagtc tttccattaa
      121 tacctgattc tctgccaaca aactactttt ctcttcctgc aatgctgcaa cctgcatatc
      181 cagttcatgg catctttgag caatttcttc ctttgctgac aaagcttcat ttagttcctc
      241 tgtagttttc ttcagctgac gatcaaggtc aacataggca tcatttccag cagagacagg
      301 agattcttta ctcatcagct cttgtgcggc tgttgcgaca acatgtgcaa cagattcctc
      361 catcatcata atggcttgga tgtactcttg cttctgttca cagttcacag cacagcctaa
      421 gatgagctga agcatccttc caagctctgc tgcatcagaa tgctccccaa taaggttcac
      481 atcaggaagg gtaaagtcat taatttgctg tcctaaaatc tcatgattat aatccaagat
      541 tccttttaaa attttcttta aattgcttat ctttagcctc caattatctc ctacttcagt
      601 tttgattctg tttagccaat tttcatcaaa atatgcagga tctatctttt gaagaacctg
      661 ggccatcaca accccattcg ttaaatcttc cacggtctgg catggtgcat ccacattaaa
      721 tgtctggatc caagtgagga ggctctcgca cagctccgcc cgctccagcg actctacgct
      781 gaacatggta ccacctccac ccccctgaaa atacagattt tcggatcctt tgtagagctc
      841 atccatgcca tgtgtaatcc cagcagcagt tacaaactca agaaggacca tgtggtcacg
      901 cttttcgttg ggatctttcg aaaggacaga ttgtg
//
