LOCUS       sequence-146516-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a triple mutant truncation of Hook3 in bacteria for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Schroeder CM, Vale RD
  TITLE     Assembly and activation of dynein-dynactin by the cargo adaptor 
            protein Hook3.
  JOURNAL   J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
  PUBMED    27482052
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-08-1- 1"; volume: "214"; 
            issue: "3"; pages: "309-18"
COMMENT     Sequence Label: pET28a-Hook3 N68A, W69A, K77A
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(803..823)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(870..890)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgctta tggactgtta gggtcttcta tagaatcaga ttgattgagt ctttccatta
      121 atacctgatt ctctgccaac aaactacttt tctcttcctg caatgctgca acctgcatat
      181 ccagttcatg gcatctttga gcaatttctt cctttgctga caaagcttca tttagttcct
      241 ctgtagtttt cttcagctga cgatcaaggt caacataggc atcatttcca gcagagacag
      301 gagattcttt actcatcagc tcttgaatgg ctgtcatgac aacatgttga acagattcct
      361 ccatcatcat aatggcttgg atgtactctt gcttctgttc acagttcaca gcacagccta
      421 agatgagctg aagcatcctt ccaagctctg ctgcatcaga atgctcccca ataaggttca
      481 catcaggaag ggtaaagtca ttaatttgct gtcctaaaat ctcatgatta taatccaaga
      541 ttccttttaa aatttttgct aaattgctta tctttagcct tgctgcatct cctacttcag
      601 ttttgattct gtttagccaa ttttcatcaa aatatgcagg atctatcttt tgaagaacct
      661 gggccatcac aaccccattc gttaaatctt ccacggtctg gcatggtgca tccacattaa
      721 atgtctggat ccaagtgagg aggctctcgc acagctccgc ccgctccagc gactctacgc
      781 tgaacatggt accacctcca cccccctgaa aatacagatt ttcggatcct ttgtagagct
      841 catccatgcc atgtgtaatc ccagcagcag ttacaaactc aagaaggacc atgtggtcac
      901 gcttttcgtt gggatctttc gaaaggacag attgtgtcga caggtaatgg ttgtctggta
      961 aaaggacag
//
