LOCUS       sequence-146520-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA expression vector for mouse Dedd gene targeting intron 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  TITLE     pUC57-sgDedd-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUC57-sgDedd-2
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    84..105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(120..125,126..143,144..150)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    158..174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     163..185
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     182..198
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     182..198
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        complement(256..331)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(353..371)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(418..435)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(418..434)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(427..449)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(643..662)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     762..784
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tctcccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
       61 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      121 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      181 acaggaaaca gctatgacca tgattacgcc aagcttgcat gcaggcctct gcagtcgacg
      241 ggccctttaa aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct
      301 tattttaact tgctatttct agctctaaaa cctgaccttt acatgaggca ctcctatagt
      361 gagtcgtatt agggatccga tatctagatg cattcgcgag gtaccgagct cgaattcact
      421 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      481 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      541 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
      601 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      661 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
      721 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      781 gaggttttca ccgtcatcac c
//
