LOCUS       sequence-146523-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pcDNA4TO-24xGCN4_v4-BFP-24xPP7
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(739..795)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(883..939)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 ttagatatcg ttcagtttgt gtcccagctt agaaggcaga tcgcagtagc gagccacagc
       61 cacctcgtgc tgttccacgt aggtctcgtt attagcctct ttgatgcgct ccaggcggta
      121 atccacgtaa tacacgcctg gcattttcag gttttttgcg ggttttttag agcggtatgt
      181 agttttaatg tttgcaatca gatggcttcc ccccaccagt ttcagtgcca tatcgttgcg
      241 cccttccagt cccccgtcag ctgggtacag agtctcagtg aatgcctccc agcccagggt
      301 cttcttctgc atcacggggc cattggaagt gaagttcacg cctctgatct tcacattata
      361 gatcaggcag ccgtcctgca ggcttgtatc ctgagtggcg gtcagcaccc cgccgtcctc
      421 gtaagttgtc acccgttccc atgtaaatcc ctcagggaag gactgtttaa agaaatcagg
      481 aattccctgg gtatggttga tgaaagtctt gcttccatac agaaagctgg tagccagaat
      541 gtcaaaagcg aaaggcaggg ggcccccctc caccactttg atcctcattg tctgtgttcc
      601 ttcatagggt ttcccctctc cttcagatgt acatttgaaa tggtgattgt ccactgtccc
      661 ctccatatac agcttcatat gcatattttc tttaatcagc tcagatgacc ggtgcggccg
      721 ctgacccgag ccagaaccct ttttaagtcg ggctacttca ttctcgagat gataattttt
      781 cgacagaagt tcctcaccac tgccacttcc cttttttaaa cgtgcaacct cattttccaa
      841 atgataatct tttgagagca gttcttcgcc tgatcctgat cctttcttca aacgtgcaac
      901 ttcgttttcc aggtggtaat tcttactgag tagttcctca cctgatccgg accccttttt
      961 aagtcttgca acctcatttt caagatgata gttcttcga
//
