LOCUS       sequence-146525-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a double mutant truncation of Hook3 in bacteria for 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Schroeder CM, Vale RD
  TITLE     Assembly and activation of dynein-dynactin by the cargo adaptor 
            protein Hook3.
  JOURNAL   J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
  PUBMED    27482052
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-08-1- 1"; volume: "214"; 
            issue: "3"; pages: "309-18"
COMMENT     Sequence Label: pET28a-Hook3 D102A, E108A
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(790..810)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(857..877)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcttatgg
       61 actgttaggg tcttctatag aatcagattg attgagtctt tccattaata cctgattctc
      121 tgccaacaaa ctacttttct cttcctgcaa tgctgcaacc tgcatatcca gttcatggca
      181 tctttgagca atttcttcct ttgctgacaa agcttcattt agttcctctg tagttttctt
      241 cagctgacga tcaaggtcaa cataggcatc atttccagca gagacaggag attctttact
      301 catcagctct tgaatggctg tcatgacaac atgttgaaca gattcctcca tcatcataat
      361 ggcttggatg tactcttgct tctgttcaca gttcacagca cagcctaaga tgagctgaag
      421 catccttcca agctctgctg catcagaatg tgccccaata aggttcactg caggaagggt
      481 aaagtcatta atttgctgtc ctaaaatctc atgattataa tccaagattc cttttaaaat
      541 tttctttaaa ttgcttatct ttagcctcca attatctcct acttcagttt tgattctgtt
      601 tagccaattt tcatcaaaat atgcaggatc tatcttttga agaacctggg ccatcacaac
      661 cccattcgtt aaatcttcca cggtctggca tggtgcatcc acattaaatg tctggatcca
      721 agtgaggagg ctctcgcaca gctccgcccg ctccagcgac tctacgctga acatggtacc
      781 acctccaccc ccctgaaaat acagattttc ggatcctttg tagagctcat ccatgccatg
      841 tgtaatccca gcagcagtta caaactcaag aaggaccatg tggtcacgct tttcgttggg
      901 atctttcgaa aggacagatt gtgtcgacag gtaatggttg tctggtaaaa
//
