LOCUS       sequence-146529-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Hook domain and first coiled coil of Hook3 in bacteria for
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Schroeder CM, Vale RD
  TITLE     Assembly and activation of dynein-dynactin by the cargo adaptor 
            protein Hook3.
  JOURNAL   J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
  PUBMED    27482052
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-08-1- 1"; volume: "214"; 
            issue: "3"; pages: "309-18"
COMMENT     Sequence Label: pET28a-Hook3 aa 1-239
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(804..824)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(871..891)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt
       61 gcggccgctt atggactgtt agggtcttct atagaatcag attgattgag tctttccatt
      121 aatacctgat tctctgccaa caaactactt ttctcttcct gcaatgctgc aacctgcata
      181 tccagttcat ggcatctttg agcaatttct tcctttgctg acaaagcttc atttagttcc
      241 tctgtagttt tcttcagctg acgatcaagg tcaacatagg catcatttcc agcagagaca
      301 ggagattctt tactcatcag ctcttgaatg gctgtcatga caacatgttg aacagattcc
      361 tccatcatca taatggcttg gatgtactct tgcttctgtt cacagttcac agcacagcct
      421 aagatgagct gaagcatcct tccaagctct gctgcatcag aatgctcccc aataaggttc
      481 acatcaggaa gggtaaagtc attaatttgc tgtcctaaaa tctcatgatt ataatccaag
      541 attcctttta aaattttctt taaattgctt atctttagcc tccaattatc tcctacttca
      601 gttttgattc tgtttagcca attttcatca aaatatgcag gatctatctt ttgaagaacc
      661 tgggccatca caaccccatt cgttaaatct tccacggtct ggcatggtgc atccacatta
      721 aatgtctgga tccaagtgag gaggctctcg cacagctccg cccgctccag cgactctacg
      781 ctgaacatgg taccacctcc acccccctga aaatacagat tttcggatcc tttgtagagc
      841 tcatccatgc catgtgtaat cccagcagca gttacaaact caagaaggac catgtggtca
      901 cgc
//
