LOCUS       sequence-146533-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector containing flag tagged S155,172D MFF 
            isoform 5 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.PGK.blast-Flag-MFF-S155,172D
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    52..76
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(804..827)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(836..860)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgagcgg ccgccactgt gctggatatc aaccactttg
       61 tacaagaaag ctgggtccta gcggcgaaac cagagccagc tattaagcag ccagaaagct
      121 acagtaattg aatacatgac catttctctt ttagcacgtt ctttgttctc ctcttccaga
      181 agttgtagac gtctatttag tttgattatc tgtcgtctta gtgaagctgc atctacaaca
      241 gtcaggtcat ctgacgttcc ttcaatggtt gtatctatat ttgaaatgcc atacctgacg
      301 ttgtcatgat gaggattaga agtggcggca gcagacccac cacgcaacac aggtagatca
      361 tcatttctga ccagctgtcc attttggcga acagcatttt cactcatatc ccgctctctt
      421 tttagtctgc caactgctcg gatttcttca ttttgagggg ttgtaggagg tctttctaaa
      481 tccagaaaat ctagtggtct ttcactcagc gtaagtacac gaggtggtgt tttcagtgcc
      541 aggggtttaa agggagttga ctgaataagg tcaagatctg ctggtcttga aaatgaaaca
      601 tcttcattat ttcctgctac aacaatcctc tccggaactt gcattatcac actagcattt
      661 ggaactcctt cttggaatcc ttgttccagg tcagcgtttg gcggtgctac ttttaacttt
      721 tctgggaccc tcattcgctg actaatgcct tcagtatatt ccatttcgta ctgaattcga
      781 ctaatttctg ccatagatcc gcccttgtca tcgtcgtcct tgtaatccat ggtgaagcct
      841 gcttttttgt acaaacttgt tgatatctgc agaattccac cacactggac tagtcgacga
      901 accggtggat ccccctgggg agagaggtcg gtgattcggt caacgaggga gc
//
