LOCUS       sequence-146541-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton , N1 Cloning Vector, Excitation:507 / 
            572, Emission:516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-MYR-N-5
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..83)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(156..359)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(185..205)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        360..663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atacggagtt tgatcttcat gtctggctta atcgcactca tggtggcgac cggtcccttt
       61 ggcttgctct tgctgcttcc catggtggcg aattcgaagc ttgagctcga gatctgagtc
      121 cggtagcgct agcggatctg acggttcact aaaccagctc tgcttatata gacctcccac
      181 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
      241 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
      301 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
      361 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
      421 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
      481 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
      541 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
      601 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
      661 acgcggaact ccatatatgg gctatgaact aatgaccccg taatt
//
