LOCUS       sequence-146548-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a truncation of Hook3 in bacteria for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Schroeder CM, Vale RD
  TITLE     Assembly and activation of dynein-dynactin by the cargo adaptor 
            protein Hook3.
  JOURNAL   J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
  PUBMED    27482052
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-08-1- 1"; volume: "214"; 
            issue: "3"; pages: "309-18"
COMMENT     Sequence Label: pET28a-Hook3 aa 1-552
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgctta tagatgttct tcaagcttct tttttagaag gactgaatct tctgcttttg
      121 agccttgatc ctgtaaagat ttttgtaatt cttcaacttg tgactgtact tccagaagtc
      181 tttgattcac cagcctattc tctgtctcca gttcattctt gcgtagattt gcatcatcta
      241 gaaggctctg caataaggct attttttcat tgtccgaacc ttcttggtta agctttaaca
      301 tcttattctc atgctgaaga cgaataagtt tctccctgat ttcaggtgta acaatctctg
      361 cagctagact gtctgaagac tcctgacttc caagaggcat taacccttgt gttgtgagct
      421 gcccttcttg agcttgtaca caacgaagct cttcaatggt ttccttcaga gaatcccttt
      481 ctgttctcag cctgtccttt tctttttgaa gactgtcaac tttttctttt agccgcttat
      541 attcaaaatc tagtttatct gctttctttg attcttcgga taatctgttt tgtagttcta
      601 ctacctgtct cttgtaggtt tcaagttgac ttcgcgctgc gttggccttt cttaactctt
      661 cctctagact gacagtattc tgcatataca tggtattctt ctcttctaag agtttaacct
      721 gccgccttaa atcaccaagg tcttctagct tctttttata agattctact tgaccttcta
      781 gtttagatac tttatcagaa gaatgtctca gcacgtcgat ctcatctttc agagactgag
      841 ct
//
