LOCUS       sequence-146551-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Kras wild-type.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     Mutant Ras Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBabe-Kras Wt
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     168..190
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     206..222
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             261..287
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             267..293
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             315..881
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
ORIGIN
        1 cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc
       61 ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt
      121 acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc
      181 tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc taggcgccgg
      241 ccggatcaat ggcttcaagt tatccatatg atgtgcccga ctatgcctca cttggaggac
      301 cctctggatc caccatgact gaatataaac ttgtggtagt tggagctggc ggcgtaggca
      361 agagtgcctt gacgatacag ctaattcaga atcattttgt ggacgaatat gatccaacaa
      421 tagaggattc ctacaggaag caagtagtaa ttgatggaga aacctgtctc ttggatattc
      481 tcgacacagc aggtcaagag gagtacagtg caatgaggga ccagtacatg aggactgggg
      541 agggctttct ttgtgtattt gccataaata atactaaatc atttgaagat attcaccatt
      601 atagagaaca aattaaaaga gttaaggact ctgaagatgt acctatggtc ctagtaggaa
      661 ataaatgtga tttgccttcc agaacagtag acacaaaaca ggctcaggac ttagcaagaa
      721 gttatggaat tccttttatt gaaacatcag caaagacaag acagggtgtt gatgatgcct
      781 tctatacatt agttcgagaa attcgaaaac ataaagaaaa gatgagcaaa gatggtaaaa
      841 agaagaaaaa gaagtcaaag acaaagtgtg taattatgta agtcgaccct gtggaatg
//
