LOCUS       sequence-146567-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal Flag-tagged NLRP3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-Flag-NLRP3
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcgcccac catggactac aaagacgatg acgataaaat
      121 cgatatcgaa ttcatgacga gtgtccgttg caagctggct cagtatctag aggaccttga
      181 agatgtggac ctcaagaaat tcaaaatgca tttggaagat tacccgcccg agaaaggctg
      241 tatcccagtc cccaggggcc agatggagaa ggcagatcac ttggatctag ccacactcat
      301 gattgacttc aatggtgagg agaaggcctg ggccatggct gtgtggatct ttgctgcgat
      361 caacaggcga gacctctggg aaaaagctaa gaaggaccag ccagagtgga atgacacgtg
      421 tacatcacat tcctctatgg tatgccagga ggacagcctt gaagaagagt ggatgggttt
      481 gctgggatat ctctcccgca tctccatttg taaaaagaag aaagattact gtaagatgta
      541 cagacgacat gtgagaagca ggttctactc tatcaaggac aggaacgcgc gtctaggtga
      601 gagtgtggac ctcaacagtc gctacacgca gctccaactg gtcaaggagc atccaagcaa
      661 gcaggagcgg gagcatgaac tcctgaccat cggccggact aaaatgcggg acagccccat
      721 gagttccctt aagctggagc tgctgtttga gcccgaggac gggcactcgg agcctgtgca
      781 cacagtggtg ttccagggag cagcaggcat cgggaaaacc atcc
//
