LOCUS       sequence-146568-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the 
            pyrin domain) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-Flag-NLRP3 1-90
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(434..452)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(435..452)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(472..489)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    478..702
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcgcccac catggactac aaagacgatg acgataaaat
      121 cgatatcgaa ttcatgacga gtgtccgttg caagctggct cagtatctag aggaccttga
      181 agatgtggac ctcaagaaat tcaaaatgca tttggaagat tacccgcccg agaaaggctg
      241 tatcccagtc cccaggggcc agatggagaa ggcagatcac ttggatctag ccacactcat
      301 gattgacttc aatggtgagg agaaggcctg ggccatggct gtgtggatct ttgctgcgat
      361 caacaggcga gacctctggg aaaaagctaa gaaggaccag ccatagctcg agcatgcatc
      421 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
      481 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      541 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
      601 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
      661 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
      721 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
      781 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      841 tcgctttctt ccc
//
