LOCUS       sequence-146571-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the 
            LRR domain) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-Flag-NLRP3 711-1034
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcgcccac catggactac aaagacgatg acgataaaat
      121 cgatatcgaa ttcgttgcct gttcttccag actggtgaac tgctgcctca cttctagctt
      181 ctgccgtggt ctcttctcaa gtctaagcac caaccggagc ctcactgaac tggacctcag
      241 tgacaatact ctgggagacc cgggcatgag ggtgctgtgt gaggcactcc agcacccagg
      301 ctgtaacatt cagagactgt ggttggggcg ctgcggactg tcccatcaat gctgcttcga
      361 catctcctct gtcctgagca gcagccagaa gctggtggag ctggacctca gtgacaatgc
      421 cctgggggac tttggaatca gattgctgtg tgtgggactg aagcacctgc tctgcaacct
      481 ccagaaactg tggttggtga gctgctgtct cacatccgcg tgttgtcagg atctcgcatt
      541 ggttctgagc tccaaccatt ctctgaccag actgtacatt ggagaaaatg ccttgggaga
      601 ctcaggagtc caagttttgt gtgaaaagat gaaggaccca cagtgtaact tgcagaagct
      661 ggggttggtg aattccggcc ttacttcaat ctgttgttca gctctgacct ctgtgctcaa
      721 aaccaaccag aacttcacac acctctatct acgaagcaat gcccttggag acacaggact
      781 caggctcctc tgtgaggggc ttctgcaccc gg
//
