LOCUS       sequence-146573-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged pro-IL-1-beta in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pCMV-pro-Il1b-C-Flag
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             876..941
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagaatt aagcttgcgg ccgcgaattc atggcaactg
       61 ttcctgaact caactgtgaa atgccacctt ttgacagtga tgagaatgac ctgttctttg
      121 aagttgacgg accccaaaag atgaagggct gcttccaaac ctttgacctg ggctgtcctg
      181 atgagagcat ccagcttcaa atctcgcagc agcacatcaa caagagcttc aggcaggcag
      241 tatcactcat tgtggctgtg gagaagctgt ggcagctacc tgtgtctttc ccgtggacct
      301 tccaggatga ggacatgagc accttctttt ccttcatctt tgaagaagag cccatcctct
      361 gtgactcatg ggatgatgat gataacctgc tggtgtgtga cgttcccatt agacaactgc
      421 actacaggct ccgagataaa caacaaaaaa gcctcgtgct gtcggaccca tatgagctga
      481 aagctctcca cctcaatgga cagaatatca accaacaagt gatattctcc atgagctttg
      541 tacaaggaga accaagcaac gacaaaatac ctgtggcctt gggcctcaaa ggaaagaatc
      601 tatacctgtc ctgtgtaatg aaagacggca cacccaccct gcagctggag agtgtggatc
      661 ccaagcaata cccaaagaag aagatggaaa aacggtttgt cttcaacaag atagaagtca
      721 agagcaaagt ggagtttgag tctgcagagt tccccaactg gtacatcagc acctcacaag
      781 cagagcacaa gcctgtcttc ctgggaaaca acagtggtca ggacataatt gacttcacca
      841 tggaatccgt gtcttcctct agaggatccc gggctgacta caaagaccat gacggtgatt
      901 ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagtgatcc c
//
