LOCUS       sequence-146574-         596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal Flag-tagged ASC1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-Flag-ASC1
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttacc atgggatacc cctacgacgt ccccgactac gccggatccg aattcatggg
      121 gcgggcacga gatgccatcc tggacgctct tgaaaacttg tcaggggatg aactcaaaaa
      181 gtccaagatg aagctgctga cagtgcaact gcgagaaggc tatgggcgca tcccacgcgg
      241 ggccctgctg cagatggacg ccatagatct cactgacaaa cttgtcagct actatctgga
      301 gtcgtatggc ttggagctca caatgactgt gcttagagac atgggcttac aggagctggc
      361 tgagcagctg caaacgacta aagaagagtc tggagctgtg gcagctgcag ccagtgtccc
      421 tgctcagagt acagccagaa caggacactt tgtggaccag cacaggcaag cactcattgc
      481 cagggtcaca gaagtggacg gagtgctgga tgctttgcat ggcagtgtgc tgactgaagg
      541 acagtaccag gcagttcgtg cagagaccac cagccaagac aagatgagga agctct
//
