LOCUS       sequence-146579-         559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that serves as the PCR template to create genomic pdt1B 
            insertions. Plasmid contains the R6K origin of replication so needs 
            pir gene to replicate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECT1B
FEATURES             Location/Qualifiers
     source          1..559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    252..285
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tcactggtga aaagaaaaac caccctggct cccaatacgc aaaccgcctc tccccgcgcg
       61 ttggccgatt cattaatgca actggcacga caggtttccc gactggaaag cgggcaggcg
      121 gcgaacaaaa acgaagaaaa caccaacgaa gtgcgcgcga tcatgctgaa cgcgggccag
      181 gcgaaccgtc ttcagttgta accgcggagg agaaagtacc atgattccgg ggatccgtcg
      241 acctgcagtt cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
      301 ctcacgctgc cgcaagcact cagggcgcaa gggctgctaa aggaagcgga acacgtagaa
      361 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
      421 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
      481 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
      541 tggtaaggtt gggaagccc
//
