LOCUS       sequence-146580-         506 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that serves as the PCR template to create genomic pdt1D 
            insertions. Plasmid contains the R6K origin of replication so needs 
            pir gene to replicate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECT1D
FEATURES             Location/Qualifiers
     source          1..506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    246..279
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gtgaaaagaa aaaccaccct ggctcccaat acgcaaaccg cctctccccg cgcgttggcc
       61 gattcattaa tgcaactggc acgacaggtt tcccgactgg aaagcgggca ggcggcgaac
      121 aaaaacgaag aaaacaccaa cgaagtggcc cccaatatgc tgaacgcggg ccaggcgaac
      181 cgtcttcagt tgtaaccgcg gaggagaaag taccatgatt ccggggatcc gtcgacctgc
      241 agttcgaagt tcctattctc tagaaagtat aggaacttca gagcgctttt gaagctcacg
      301 ctgccgcaag cactcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag
      361 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
      421 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
      481 ctgggcggtt ttatggacag caagcg
//
