LOCUS       sequence-146582-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that serves as the PCR template to create genomic pdt1 
            insertions. Plasmid contains the R6K origin of replication so needs 
            pir gene to replicate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECT1
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    301..334
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga gaatcagctg ttgcccgtct cactggtgaa
       61 aagaaaaacc accctggctc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      121 attaatgcaa ctggcacgac aggtttcccg actggaaagc gggcaggcgg cgaacaaaaa
      181 cgaagaaaac accaacgaag tgccgacctt tatgctgaac gcgggccagg cgaaccgtct
      241 tcagttgtaa ccgcggagga gaaagtacca tgattccggg gatccgtcga cctgcagttc
      301 gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaagc tcacgctgcc
      361 gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc
      421 agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg
      481 caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg
      541 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
      601 ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg
      661 gatcaagatc tgatcaagag acaggatgag gatcgttt
//
