LOCUS       sequence-146584-         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that serves as the PCR template to create genomic pdt3 
            insertions. Plasmid contains the R6K origin of replication so needs 
            pir gene to replicate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECT3
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    253..286
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ctcactggtg aaaagaaaaa ccaccctggc tcccaatacg caaaccgcct ctccccgcgc
       61 gttggccgat tcattaatgc aactggcacg acaggtttcc cgactggaaa gcgggcaggc
      121 ggcgaacaaa aacgaagaaa acaccaacga agtgccgacc tttatgctga acgcgggcca
      181 ggcgaacaga agacgagttt aaccgcggag gagaaagtac catgattccg gggatccgtc
      241 gacctgcagt tcgaagttcc tattctctag aaagtatagg aacttcagag cgcttttgaa
      301 gctcacgctg ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga
      361 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga
      421 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat
      481 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct
      541 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc ttt
//
