LOCUS       sequence-146588-         494 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Toggle switch plasmid containing lacI-pdt3D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECJ3D
FEATURES             Location/Qualifiers
     source          1..494
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(33..58,59..64,65..81,82..87,88..106))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    63..81
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtctatgcta tgctgatcta caactggcat gcggtcagtg cgtcctgctg atgtgctcag
       61 tatctctatc actgataggg atgtcaatct ctatcactga tagggaaacg tttcgcagaa
      121 gcttccgcaa ggtaccactt tgccgcggtt aaactcgtct tctgttcgcc tggcccgcgt
      181 tcagcatatt gggggccact tcgttggtgt tttcttcgtt tttgttcgcc gcctgcccgc
      241 tttccagtcg ggaaacctgt cgtgccagtt gcattaatga atcggccaac gcgcggggag
      301 aggcggtttg cgtattggga gccagggtgg tttttctttt caccagtgag acgggcaaca
      361 gttgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
      421 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
      481 cttcggtatc gtcg
//
