LOCUS       sequence-146593-         470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Toggle switch plasmid containing lacI-pdt3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECJ3
FEATURES             Location/Qualifiers
     source          1..470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    51..69
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtgcgtcctg ctgatgtgct cagtatctct atcactgata gggatgtcaa tctctatcac
       61 tgatagggaa acgtttcgca gaagcttccg caaggtacca ctttgccgcg gttaaactcg
      121 tcttctgttc gcctggcccg cgttcagcat aaaggtcggc acttcgttgg tgttttcttc
      181 gtttttgttc gccgcctgcc cgctttccag tcgggaaacc tgtcgtgcca gttgcattaa
      241 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggagccaggg tggtttttct
      301 tttcaccagt gagacgggca acagttgatt gcccttcacc gcctggccct gagagagttg
      361 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa
      421 cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg
//
