LOCUS       sequence-146627-         473 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive expression of gfp-pdt3E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pZE27GFP3E
FEATURES             Location/Qualifiers
     source          1..473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     184..202
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(337..365)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
       61 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
      121 ggcgccccag ctggcaattc cgacgtctaa gaaaccatta ttatcatgac attaacctat
      181 aaaaataggc gtatcacgag gccctttcgt cttcacctcg agtggtgcaa aacctttcgc
      241 ggtatggcat gatagcgccc ggaagagagt caattcaggg aattcattaa agaggacaaa
      301 ggtaccatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      361 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      421 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tcc
//
