LOCUS       sequence-146643-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin variant with mutations L69P and V70L reverted to wild type
            (i53-DM mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Canny MD, Moatti N, Wan LCK, Fradet-Turcotte A, Krasner D, 
            Mateos-Gomez PA, Zimmermann M, Orthwein A, Juang YC, Zhang W, 
            Noordermeer SM, Seclen E, Wilson MD, Vorobyov A, Munro M, Ernst A, 
            Ng TF, Cho T, Cannon PM, Sidhu SS, Sicheri F, Durocher D
  TITLE     Inhibition of 53BP1 favors homology-dependent DNA repair and 
            increases CRISPR-Cas9 genome-editing efficiency.
  JOURNAL   Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 
            2017 Nov 27.
  PUBMED    29176614
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4021"; 
            journalName: "Nat Biotechnol"; date: "2018-01"; volume: "36"; issue:
            "1"; pages: "95-102"
COMMENT     Sequence Label: pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..100
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(399..417)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(400..417)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(437..454)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    443..667
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accatggact acaaagacga tgacgataaa gccgccagtt taaacggcgc
      121 gccattaatt aaggatccaa tgttgatttt cgtgaaaacc cttaccggga aaaccatcac
      181 cctcgaggtt gaaccctcgg atacgataga aaatgtaaag gccaagatcc aggataagga
      241 aggaattcct cctgatcagc agagactggc ctttgctggc aaatcgctgg aagatggacg
      301 tactttgtct gactacaata ttctaaagga ctctaaactt catctagtgt tgagacttcg
      361 ttgagcggcc gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct
      421 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
      481 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
      541 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
      601 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
      661 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
      721 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      781 gccagcgcc
//
