LOCUS       sequence-146646-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing Eps8 fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Menna E, Disanza A, Cagnoli C, Schenk U, Gelsomino G, Frittoli E, 
            Hertzog M, Offenhauser N, Sawallisch C, Kreienkamp HJ, Gertler FB, 
            Di Fiore PP, Scita G, Matteoli M
  TITLE     Eps8 regulates axonal filopodia in hippocampal neurons in response 
            to brain-derived neurotrophic factor (BDNF).
  JOURNAL   PLoS Biol. 2009 Jun 30;7(6):e1000138. doi: 
            10.1371/journal.pbio.1000138. Epub 2009 Jun 30.
  PUBMED    19564905
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2009-06-30"; volume: "7(6):e1000138. doi"; pages: " 
            10.1371/journal.pbio.1000138. Epub 2009 Jun 30"
COMMENT     Sequence Label: pRRLsin.PPT.CMV­‐GFP­‐Eps8 WT
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             455..688
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    760..881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(797..816)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     851..870
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcgggcgcag tgctgcgcag aggaagttcc acgtgccacg gcagaacgtt ccagtgatca
       61 atatcactta tgactcctca ccggaagaag taaagacttg gctgcagtca aagggattca
      121 atcccgtgac tgtcaatagc ctcggggtgt tgaacggagc acaactcttt tctctcaaca
      181 aagacgaact gaggtctgtc tgcccggaag gtgccagagt ctttaaccaa atcactgttc
      241 agaaagctgc tttggaggac agtaatggaa gctccgagtt acaagagatc atgcggagac
      301 ggcaggagaa gatcagcgcc gctgcgagcg actcgggagt ggagtctttt gatgaaggga
      361 gcagccactg agaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
      421 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
      481 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
      541 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      601 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      661 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
      721 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
      781 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      841 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
      901 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      961 cccatggctg actaattttt tttatttatg ca
//
