LOCUS       sequence-146651-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For generating mRNA for zebrafish injection of cytoplasmic 
            fluorogen-activating protein (FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   He J, Wang Y, Missinato MA, Onuoha E, Perkins LA, Watkins SC, St 
            Croix CM, Tsang M, Bruchez MP
  TITLE     A genetically targetable near-infrared photosensitizer.
  JOURNAL   Nat Methods. 2016 Mar;13(3):263-268. doi: 10.1038/nmeth.3735. Epub 
            2016 Jan 25.
  PUBMED    26808669
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3735"; journalName: "Nat Methods"; date: "2016-03"; 
            volume: "13"; issue: "3"; pages: "263-268"
COMMENT     Sequence Label: pCS2+ MBIC5-mCer3
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..96
                     /gene="bla"
                     /label=AmpR promoter
     CDS             281..659
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(315..334)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
       61 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      121 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
      181 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      241 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
      301 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
      361 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
      421 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
      481 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
      541 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
      601 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
      661 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
      721 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
      781 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
      841 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagt
//
