LOCUS       sequence-146653-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter driving synthetic optimised GUSPlus gene. Can be 
            used as a reporter of promoter activity in plants by replacing Ubi 
            promoter with sequence of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Vickers CE, Xue GP, Gresshoff PM
  TITLE     A synthetic xylanase as a novel reporter in plants.
  JOURNAL   Plant Cell Rep. 2003 Sep;22(2):135-40. Epub 2003 Jul 4.
  PUBMED    12845475
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2003-09"; volume: "22(2)"; pages: "135-40. Epub 2003 
            Jul 4"
COMMENT     Sequence Label: pUbiGUSPlus
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(1..252)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(284..301)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
       61 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
      121 aaataacgtc atgcattaca tgttgattat tacatgctta acgtaattca acagaaatta
      181 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
      241 gtttgaacga tcggggaaat tcgagctggt caccaattca cacgtgatgg tgatggtgat
      301 ggctagcgtt cttgtagccg aaatctggaa tgttggtcca gcgctcgcga aagacgtgcg
      361 cggcgagctt cggcttgcgg tcacgagtga acacgccctt cttgtttcct tggacgcgca
      421 tcacgccctg agaggtcgcg aagtccgcga agttccacgc ttgctcaccc acgaagttct
      481 caaactcatc gaacacgacg tggttcgcct ggtagtactc gacttgatat tcctcggtga
      541 acatcactgg atcaatgtcg tgaaagcccg caacggtgtc tgcgccgtac tcagtgatca
      601 tgatcggctt tcctgggcaa cgcttgttcc acgcgtgaaa ttcctggcgg agatggactt
      661 tggccgcttc gagatcaccg ccatcgaagt accatccgtt atagcgattg agcgcgatga
      721 cgtcaatcag ttcggcgact ttgtccgtct ccggggtagc catcacaaac agcacgatcg
      781 tgaccggacg cttctgtggg tcgagttcct tggtcagctc caccaacggc ttgaagtact
      841 cgtacgcgcc ctcttcctca gtcgccgcct cgttggcgat gctccacatc acga
//
