LOCUS       sequence-146656-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pHAGE-TO-dCas9
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..25
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     35..59
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      152..161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             164..184
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
       61 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      121 taccggttaa ggtgaacgcg tgcaggctgg cgccaccatg gcccctaaaa agaaacgcaa
      181 ggtcgaattc ggaagcggaa gcgacaagaa gtactccatt gggctcgcta tcggtaccaa
      241 cagcgtcggc tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt
      301 tctgggcaat accgatcgcc acagcataaa gaagaacctc attggagccc tcctgttcga
      361 ctccggggag acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg
      421 cagaaagaat cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga
      481 tgactctttc ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga
      541 gcgccaccca atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac
      601 catatatcat ctgaggaaga agctggtaga cagtactgat aaggctgact tgcggttgat
      661 ctatctcgcg ctggcgcaca tgatcaaatt tcggggacac ttcctcatcg agggggacct
      721 gaacccagac aacagcgatg tcgacaaact ct
//
