LOCUS       sequence-146659-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCP-3XBFPnls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pHAGE-TO-MCP-3XBFPnls
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..22
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     32..56
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct
       61 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctac
      121 cggtggaagt gggcgcgcca ccatggcttc taactttact cagttcgttc tcgtcgacaa
      181 tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc gctaacggga tcgctgaatg
      241 gatcagctct aactcgcgtt cacaggctta caaagtaacc tgtagcgttc gtcagagctc
      301 tgcgcagaat cgcaaataca ccatcaaagt cgaggtgcct aaaggcgcct ggcgttcgta
      361 cttaaatatg gaactaacca ttccaatttt cgccacgaat tccgactgcg agcttattgt
      421 taaggcaatg caaggtctcc taaaagatgg aaacccgatt ccctcagcaa tcgcagcaaa
      481 ctccggcatc tacactagtg gctctggatc cggaagtatg gtgtctaagg gcgaagagct
      541 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
      601 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
      661 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
      721 cggcagcaag accttcatca accacac
//
