LOCUS       sequence-146660-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCP-3XBFPnls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pHAGE-EFS-MCP-3XBFPnls
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttaaggtga acgcgtgcag gctggcgcca ccatggcttc taactttact cagttcgttc
       61 tcgtcgacaa tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc gctaacggga
      121 tcgctgaatg gatcagctct aactcgcgtt cacaggctta caaagtaacc tgtagcgttc
      181 gtcagagctc tgcgcagaat cgcaaataca ccatcaaagt cgaggtgcct aaaggcgcct
      241 ggcgttcgta cttaaatatg gaactaacca ttccaatttt cgccacgaat tccgactgcg
      301 agcttattgt taaggcaatg caaggtctcc taaaagatgg aaacccgatt ccctcagcaa
      361 tcgcagcaaa ctccggcatc tacgggggat ctggtagcgg cgctggatcc ggaagtatgg
      421 tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca
      481 ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
      541 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
      601 tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg
      661 acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag
      721 ac
//
