LOCUS       sequence-146663-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N22p-3XRFPnls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pHAGE-EFS-N22p-3XRFPnls
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(279..297)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     547..566
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aggtgaacgc gtgcaggctg gcgccaccat gggtaatgct cggacccggc gaagagagag
       61 gcgggctgag aagcaggcac agtggaaggc tgcaaacggt ggcggaggta ctagtggctc
      121 tggatccgga agtatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
      181 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      241 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      301 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
      361 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      421 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      481 ctcactgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      541 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      601 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
      661 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      721 cgcctacaac gtcaacatca a
//
