LOCUS       sequence-146666-         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for mammalian expression of HCMV protein UL136. Expresses 
            only the 19kda isoform of UL136. Also expresses EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F
  TITLE     Complex expression of the UL136 gene of human cytomegalovirus 
            results in multiple protein isoforms with unique roles in 
            replication.
  JOURNAL   J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 
            2014 Oct 8.
  PUBMED    25297993
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02711-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "24"; pages: "14412-25"
COMMENT     Sequence Label: pCIG1_UL136_19kDa_EE
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    29..581
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    31..598
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(191..210)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     420..437
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tcgcccttgc tcaccatggt tgtggccata ttatcatcgt gtttttcaaa ggaaaaccac
       61 gtccccgtgg ttcggggggc ctagacgttt ttttaacctc gactaaacac atgtaaagca
      121 tgtgcaccga ggccccagat cagatcccat acaatggggt accttctggg catccttcag
      181 ccccttgttg aatacgcttg aggagagcca tttgactctt tccacaacta tccaactcac
      241 aacgtggcac tggggttgtg ccgcctttgc aggtgtatct tatacacgtg gcttttggcc
      301 gcagaggcac ctgtcgccag gtggggggtt ccgctgcctg caaagggtcg ctacagacgt
      361 tgtttgtctt caagaagctt ccagaggaac tgcttccttc acgacattca acagaccttg
      421 cattcctttg gcgagagggg aaagacccct aggaatgctc gtcaagaaga cagggccagg
      481 tttccgggcc ctcacattgc caaaagacgg caatatggtg gaaaataaca tatagacaaa
      541 cgcacaccgg ccttattcca agcggcttcg gccagtaacg ttaggggggg gggagggaga
      601 ggg
//
