LOCUS       sequence-146673-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual-fluorescent surrogate reporter vector for in vitro functional 
            assay of programable DNA nuclease and enrichment of genetically 
            edited cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Zhou Y, Liu Y, Hussmann D, Brogger P, Al-Saaidi RA, Tan S, Lin L, 
            Petersen TS, Zhou GQ, Bross P, Aagaard L, Klein T, Ronn SG, Pedersen
            HD, Bolund L, Nielsen AL, Sorensen CB, Luo Y
  TITLE     Enhanced genome editing in mammalian cells with a modified 
            dual-fluorescent surrogate system.
  JOURNAL   Cell Mol Life Sci. 2016 Jan 11.
  PUBMED    26755436
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2016 Jan 11."
COMMENT     Sequence Label: P119 C-Check
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..27
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(274..293)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     621..642
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    810..931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(847..866)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     901..920
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc ttggtctcaa
       61 ccgtccagcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      121 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      181 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      241 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      301 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      361 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      421 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      481 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      541 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
      601 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      661 ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata
      721 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      781 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      841 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      901 gtggtttgtc caaactcatc aatgtatctt aa
//
