LOCUS       sequence-146676-         342 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector with ubiquitin variant with reverted mutations 
            L69P and V70L (i53-DM mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Canny MD, Moatti N, Wan LCK, Fradet-Turcotte A, Krasner D, 
            Mateos-Gomez PA, Zimmermann M, Orthwein A, Juang YC, Zhang W, 
            Noordermeer SM, Seclen E, Wilson MD, Vorobyov A, Munro M, Ernst A, 
            Ng TF, Cho T, Cannon PM, Sidhu SS, Sicheri F, Durocher D
  TITLE     Inhibition of 53BP1 favors homology-dependent DNA repair and 
            increases CRISPR-Cas9 genome-editing efficiency.
  JOURNAL   Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 
            2017 Nov 27.
  PUBMED    29176614
REFERENCE   2  (bases 1 to 342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4021"; 
            journalName: "Nat Biotechnol"; date: "2018-01"; volume: "36"; issue:
            "1"; pages: "95-102"
COMMENT     Sequence Label: pMX-HA-UbvG08_MUT-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..342
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..70
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcctctagac tgccggatct agctagttaa ttaagccacc atgtacccat acgatgttcc
       61 agattacgct gcagctgcaa tgttgatttt cgtgaaaacc cttaccggga aaaccatcac
      121 cctcgaggtt gaaccctcgg atacgataga aaatgtaaag gccaagatcc aggataagga
      181 aggaattcct cctgatcagc agagactggc ctttgctggc aaatcgctgg aagatggacg
      241 tactttgtct gactacaata ttctaaagga ctctaaactt catctagtgt tgagacttcg
      301 ttgagcggcc gctacgtaaa ttccgccccc cccccccctc tc
//
