LOCUS       sequence-146685-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Sp dCas9-3XmCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-dCas9-3XmCherry
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..31
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             170..190
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      121 ccagcctacc ggttaaggtg aacgcgtgca ggctggcgcc accatggccc ctaaaaagaa
      181 acgcaaggtc gaattcggaa gcggaagcga caagaagtac tccattgggc tcgctatcgg
      241 taccaacagc gtcggctggg ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt
      301 caaagttctg ggcaataccg atcgccacag cataaagaag aacctcattg gagccctcct
      361 gttcgactcc ggggagacgg ccgaagccac gcggctcaaa agaacagcac ggcgcagata
      421 tacccgcaga aagaatcgga tctgctacct gcaggagatc tttagtaatg agatggctaa
      481 ggtggatgac tctttcttcc ataggctgga ggagtccttt ttggtggagg aggataaaaa
      541 gcacgagcgc cacccaatct ttggcaatat cgtggacgag gtggcgtacc atgaaaagta
      601 cccaaccata tatcatctga ggaagaagct ggtagacagt actgataagg ctgacttgcg
      661 gttgatctat ctcgcgctgg cgcacatgat caaatttcgg ggacacttcc tcatcgaggg
      721 ggacctgaac ccagacaaca gcgatgtcga caaactcttt atccaactgg ttcagactta
      781 caatcagctt ttcgaggaga acccgatcaa cgcatccggc gttgacgcca aagcaatc
//
