LOCUS       sequence-146686-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Sp dCas9-3XmCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-dCas9-3XmCherry
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..742)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(310..329)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     579..597
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ccagaggttg attatctaga ttaccctact cgagcttgta cagctcgtcc atgccgccgg
       61 tggagtggcg gccctcagcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
      121 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
      181 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
      241 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
      301 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
      361 tcaccttgta gatgaactcg ccgtcctgca gtgaggagtc ctgggtcacg gtcaccacgc
      421 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
      481 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
      541 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
      601 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
      661 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
      721 cctcctcgcc cttgctcacc atgctaccat cgatgctacc cttgtacagc tcgtccatgc
      781 cgccggtgga gtggcggccc tcagcgcgtt cgtactgttc cacgatggtg tagtcctcgt
      841 tgtggg
//
