LOCUS       sequence-146687-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression flag-GFP 1-10 tetra Cys in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-GFP 1-10 tetra Cys
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             694..711
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 atggactaca aggacgacga tgacaagggg tcgaccatgg tttcgaaagg cgaggagctg
       61 ttcacaggcg tggtgccaat cctggtggag ctggacggcg acgtgaacgg ccacaaattc
      121 agcgtgagag gcgagggcga gggcgacgcc acaatcggca aactgacact gaaattcatc
      181 tgcacaacag gcaaactgcc agtgccctgg ccaacactag tgacaacact gacatacggc
      241 gtgcagtgct tcagcagata tccggaccac atgaaaagac acgacttctt caaaagcgcc
      301 atgccagagg gctacgtgca ggagagaaca atcagcttca aagacgacgg caaatacaaa
      361 acaagagccg tggtgaaatt cgagggcgac acactggtga acagaatcga gctgaaaggc
      421 acagacttca aagaggacgg caacatcctg ggccacaaac tggagtacaa cttcaacagc
      481 cacaacgtgt acatcacagc caacaaacag aaaaacggca tcaaagccaa cttcacagtg
      541 agacacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca gaacacacca
      601 atcggcgacg gcccagtgct gctgccagac aaccactacc tgagcacaca gacagtgctg
      661 agcaaagacc caaacgagaa aggcagcggc ggctgctgcc ctggttgctg ctaa
//
