LOCUS       sequence-146694-       10100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible
            manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 10100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: FUW TetO HA-AMPKa2 AS
FEATURES             Location/Qualifiers
     source          1..10100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2723
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2832..2858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     misc_feature    4561..5149
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4614..4634)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5032..5043)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5152..5168)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5153..5169)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5674..5854
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5880..5897)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5886..6110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6156..6584
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6243..6262)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6453..6474
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6593..6613)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6598..6927
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6778..6913
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6840..6859
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6975..7022
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7041..7415
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7545..7666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7582..7601)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7636..7655
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7715..7731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7715..7731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7728..7750)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7739..7755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7763..7769,7770..7787,7788..7793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7808..7829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7946..7963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8117..8705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8197..8216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8876..9667,9668..9736))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9499..9518
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9737..9841)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9916..9935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
     2821 tcgaaaccat gtatgatgtt cctgattatg ctagcctccc ggggaattcg gctgagaagc
     2881 agaagcacga cgggcgggtg aagatcggac actacgtgct gggcgacacg ctgggcgtcg
     2941 gcaccttcgg caaagtgaag attggagaac atcaattaac aggccataaa gtggcagtta
     3001 aaatcttaaa tagacagaag attcgcagtt tagatgttgt tggaaaaata aaacgagaaa
     3061 ttcaaaatct aaaactcttt cgtcatcctc atattatcaa actataccag gtgatcagca
     3121 ctccaacaga tttttttatg gtaggggaat atgtgtctgg aggtgaatta tttgactaca
     3181 tctgtaagca tggacgggtt gaagagatgg aagccaggcg gctctttcag cagattctgt
     3241 ctgctgtgga ttactgtcat aggcatatgg ttgttcatcg agacctgaaa ccagagaatg
     3301 tcctgttgga tgcacacatg aatgccaaga tagccgattt cggattatct aatatgatgt
     3361 cagatggtga atttctgaga actagttgcg gatctccaaa ttatgcagca cctgaagtca
     3421 tctcaggcag attgtatgca ggtcctgaag ttgatatctg gagctgtggt gttatcttgt
     3481 atgctcttct ttgtggcacc ctcccatttg atgatgagca tgtacctacg ttatttaaga
     3541 agatccgagg gggtgtcttt tatatcccag aatatctcaa tcgttctgtc gccactctcc
     3601 tgatgcatat gctgcaggtt gacccactga aacgagcaac tatcaaagac ataagagagc
     3661 atgaatggtt taaacaagat ttgcccagtt acttatttcc tgaagaccct tcctatgatg
     3721 ctaacgtcat tgatgatgag gctgtgaaag aagtgtgtga aaaatttgaa tgtacagaat
     3781 cagaagtaat gaacagttta tatagtggtg accctcaaga ccagcttgca gtggcttatc
     3841 atcttatcat tgacaatcgg agaataatga accaagccag tgagttctac ctcgcctcta
     3901 gtcctccatc tggttctttt atggatgata gtgccatgca tattccccca ggcctgaaac
     3961 ctcatccaga aaggatgcca cctcttatag cagacagccc caaagcaaga tgtccattgg
     4021 atgcactgaa tacgactaag cccaaatctt tagctgtgaa aaaagccaag tggcatcttg
     4081 gaatccgaag tcagagcaaa ccgtatgaca ttatggctga agtttaccga gctatgaagc
     4141 agctggattt tgaatggaag gtagtgaatg cataccatct tcgtgtaaga agaaaaaatc
     4201 cagtgactgg caattacgtg aaaatgagct tacaacttta cctggttgat aacaggagct
     4261 atcttttgga ctttaaaagc attgatgatg aagtagtgga gcagagatct ggttcctcaa
     4321 cacctcagcg ttcctgttct gctgctggct tacacagacc aagatcaagt tttgattcca
     4381 caactgcaga gagccattca ctttctggct ctctcactgg ctctttgacc ggaagcacat
     4441 tgtcttcagt ttcacctcgc ctgggcagtc acaccatgga tttttttgaa atgtgtgcca
     4501 gtctgattac tactttagcc cgttaattaa gctagctaat tcgatatcaa gcttatcgat
     4561 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4621 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4681 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4741 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4801 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4861 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4921 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4981 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5041 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5101 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     5161 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     5221 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     5281 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5341 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     5401 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     5461 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     5521 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     5581 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     5641 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     5701 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5761 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5821 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     5881 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5941 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6001 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6061 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6121 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6181 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6241 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6301 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6361 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6421 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6481 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6541 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     6601 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6661 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6721 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6781 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6841 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     6901 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     6961 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     7021 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     7081 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     7141 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     7201 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     7261 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     7321 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     7381 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     7441 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7501 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7681 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7741 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7801 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7861 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7921 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7981 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8041 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8281 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8521 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9721 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9781 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9841 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     9901 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     9961 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
    10021 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
    10081 cgatgtacgg gccagatata
//
