LOCUS       sequence-146697-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 + sgGFP-2 with blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: PXPR007 sgGFP-2
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        36..111
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    159..276
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        345..556
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      576..585
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             585..608
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             615..635
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaggacgaaa caccgggtgc ccatcctggt cgagcgtttt agagctagaa atagcaagtt
       61 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgaa
      121 ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
      181 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
      241 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
      301 gatccacttt ggcgccggct cgagtggctc cggtgcccgt cagtgggcag agcgcacatc
      361 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
      421 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
      481 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
      541 tgccgccaga acacaggtgt cgtgacgcgg gatccgccac catggattac aaagacgatg
      601 acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
      661 acaagaagta cagcatcggc ctggacatcg gcaccaa
//
