LOCUS       sequence-146707-        1846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor. To monitor Src kinase activity in mammalian cells by
            FRET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ouyang M, Sun J, Chien S, Wang Y
  TITLE     Determination of hierarchical relationship of Src and Rac at 
            subcellular locations with FRET biosensors.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14353-8. doi: 
            10.1073/pnas.0807537105. Epub 2008 Sep 17.
  PUBMED    18799748
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0807537105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-09-23- 23"; volume: "105"; issue: "38"; pages: "14353-8"
COMMENT     Sequence Label: Kras-Src FRET biosensor
FEATURES             Location/Qualifiers
     source          1..1846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..526)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(53..74)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(314..333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1082..1795
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
ORIGIN
        1 ggatcctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actatatcag ccacaacgtc tatatcaccg ccgacaagca
      481 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaaggaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc ccgcatgcat tggtattttg ggaagatcac
      721 tcgtcgggag tccgagcggc tgctgctcaa ccccgaaaac ccccggggaa ccttcttggt
      781 ccgggagagc gagacgacaa aaggtgccta ttgcctctcc gtttctgact ttgacaacgc
      841 caaggggctc aatgtgaagc actacaagat ccgcaagctg gacagcggcg gcttctacat
      901 cacctcacgc acacagttca gcagcctgca gcagctggtg gcctactact ccaaacatgc
      961 tgatggcttg tgccaccgcc tgaccaacgt ctgcgggtct acatctggat ctgggaagcc
     1021 gggttctggt gagggttctt ggatggagga ctatgactac gtccacctac agggggagct
     1081 catgtctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg
     1141 tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacgtacgg
     1201 taaattgacc ttaaaattac tctgtactac tggtaaattg ccagttccat ggccaacctt
     1261 agtcactact ttaggttatg gtgttcaatg ttttgctaga tacccagatc atatgaaaca
     1321 acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt
     1381 caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt
     1441 taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa
     1501 attggaatac aactataact ctcacaatgt ttacatcact gctgacaaac aaaagaatgg
     1561 tatcaaagct aacttcaaaa ttagacacaa cattgaagat ggtggtgttc aattagctga
     1621 ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta
     1681 cttatcctat caatctgcct tattcaaaga tccaaacgaa aagagagacc acatggtctt
     1741 gttagaattt ttgactgctg ctggtattac cgagggtatg aatgaattgt acaaaaagaa
     1801 gaagaagaag aagagcaaga ccaagtgcgt gatcatgtaa gaattc
//
