LOCUS       sequence-146716-         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin variant with mutations L69P and V70L reverted to wild type
            (i53-DM mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Canny MD, Moatti N, Wan LCK, Fradet-Turcotte A, Krasner D, 
            Mateos-Gomez PA, Zimmermann M, Orthwein A, Juang YC, Zhang W, 
            Noordermeer SM, Seclen E, Wilson MD, Vorobyov A, Munro M, Ernst A, 
            Ng TF, Cho T, Cannon PM, Sidhu SS, Sicheri F, Durocher D
  TITLE     Inhibition of 53BP1 favors homology-dependent DNA repair and 
            increases CRISPR-Cas9 genome-editing efficiency.
  JOURNAL   Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 
            2017 Nov 27.
  PUBMED    29176614
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4021"; 
            journalName: "Nat Biotechnol"; date: "2018-01"; volume: "36"; issue:
            "1"; pages: "95-102"
COMMENT     Sequence Label: pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(238..261)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(283..302)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(284..302)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(347..550)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(376..396)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tttagagtcc tttagaatat tgtagtcaga caaagtacgt ccatcttcca gcgatttgcc
       61 agcaaaggcc agtctctgct gatcaggagg aattccttcc ttatcctgga tcttggcctt
      121 tacattttct atcgtatccg agggttcaac ctcgagggtg atggttttcc cggtaagggt
      181 tttcacgaaa atcaacattg gatccttaat taatggcgcg ccgtttaaac tggcggcttt
      241 atcgtcatcg tctttgtagt ccatggtacc aagcttgggt ctccctatag tgagtcgtat
      301 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      361 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      421 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
      481 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
      541 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
      601 cccataaggt catgtactgg
//
