LOCUS       sequence-146724-        1180 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: 3xFLAG-dCas9/pMXs-IG
FEATURES             Location/Qualifiers
     source          1..1180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..160
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatctagct agttaattaa
       61 ggatccgttt agtgaaccgt cagaattaac catggactac aaagaccatg acggtgatta
      121 taaagatcat gacatcgatt acaaggatga cgatgacaag ctcatggaca agaagtactc
      181 cattgggctc gctatcggca caaacagcgt cggctgggcc gtcattacgg acgagtacaa
      241 ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca taaagaagaa
      301 cctcattggc gccctcctgt tcgactccgg ggagacggcc gaagccacgc ggctcaaaag
      361 aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc aggagatctt
      421 tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg agtccttttt
      481 ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg tggacgaggt
      541 ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg tagacagtac
      601 tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca aatttcgggg
      661 acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca aactctttat
      721 ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg catccggagt
      781 tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg aaaacctcat
      841 cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg ccctgtcact
      901 cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca agcttcaact
      961 gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg gcgaccagta
     1021 cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgctga gtgatattct
     1081 gcgagtgaac acggagatca ccaaagctcc gctgagcgct agtatgatca agcgctatga
     1141 tgagcaccac caagacttga ctttgctgaa ggcccttgtc
//
