LOCUS       sequence-146735-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: 3xFLAG-dCas9/pMXs-neo
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..825)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(142..161)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     752..771
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcgtcgacga tgcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
       61 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
      121 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
      181 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
      241 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc
      301 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
      361 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
      421 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
      481 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
      541 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
      601 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc
      661 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
      721 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
      781 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
      841 tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
      901 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
      961 cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca
     1021 agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     1081 attcatccgg gg
//
