LOCUS       sequence-146754-        2750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSB3K3 carrying J114-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW202J114-gfp
FEATURES             Location/Qualifiers
     source          1..2750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..21
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(239..1054)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(239..1054)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     962..981
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1046..1065
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(1649..2193)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1729..1748)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2661..2679
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2683..2726)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2729..2750
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct
       61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg
      181 agtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
      241 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
      301 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
      361 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
      421 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
      481 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
      541 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
      601 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
      661 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
      721 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
      781 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
      841 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
      901 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
      961 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     1021 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     1081 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     1141 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
     1201 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
     1261 ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg
     1321 atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc
     1381 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt
     1441 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg
     1501 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     1561 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg
     1621 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc
     1681 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
     1741 cgtttcccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     1801 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     1861 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     1921 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     1981 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     2041 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     2101 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     2161 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa ggggtctgac
     2221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2581 ttatccgcct ccatccagtc tattccatgg tgccacctga cgtctaagaa accattatta
     2641 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     2701 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
//
