LOCUS       sequence-146779-        5806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. Coli/Gram-positive Bacterial Shuttle Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Dunny GM, Lee LN, LeBlanc DJ
  TITLE     Improved electroporation and cloning vector system for gram-positive
            bacteria.
  JOURNAL   Appl Environ Microbiol. 1991 Apr;57(4):1194-201.
  PUBMED    1905518
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "1991-04"; volume: "57(4)"; pages: 
            "1194-201"
COMMENT     Sequence Label: pDL276
FEATURES             Location/Qualifiers
     source          1..5806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    257..278
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(293..298,299..316,317..323)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    331..347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     336..358
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     355..371
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     355..371
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             367..765
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGECRELFTTPRIGANQFLRRTVNAQTNPWQ
                     NISIASAISSSRTRRISAVLADERRFSA"
     misc_feature    384..440
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(441..458)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(441..457)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(450..472)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      931..1017
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1109..1136
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      1429..1515
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1607..1634
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      5037..5625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5526..5545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5779..5796
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagaaata
       61 cgtttcccat ttagccgtca tttcagcact cgttaaaagg tgctgacttt caactgcctg
      121 gcacaataat tttccttttt cagttacaac aagctccaat acgcaaaccg cctctccccg
      181 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      241 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      301 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      361 acagctatga ccatgattac gccaagcttg catgcctgca ggtcgactct agaggatccc
      421 cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      481 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      541 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgccgg
      601 gaattattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
      661 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
      721 cgcatctcgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
      781 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
      841 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
      901 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
      961 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     1021 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     1081 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     1141 tttctacaaa ctcttcctgt cgtcatatct acaagccatc cccccacaga tacggtaaac
     1201 tagcctcgtt tttgcatcag gaaagcagct gttttggcgg atgagagaag attttcagcc
     1261 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     1321 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     1381 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     1441 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1501 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     1561 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     1621 acggatggcc tttttgcgtt tctacaaact cttcctgtcg tcatatctac aagccatccc
     1681 cccacagata cggtaaacta gcctcgtttt tgcatcagga aagcagtcgg gcagcgttgg
     1741 gattctgaga cctctgagag gctctcagag ctatctaaag ctgagggata tataaatacc
     1801 ttagaaaatt attcgaagag cttagaagcg aaaatagagc gtttagagcg cgaggggctg
     1861 aaattagaaa aactaaaaac acaaatagct gacctaaaaa tcatgtctga gaaagaacta
     1921 gcggctatta cccctaaaaa aggcgtgttc ggtaaagaat atgtggaatt gactaaagag
     1981 cagtttgaag aatttaaagg gctgatatac cgaagcagaa accttgttca tcaaaaagag
     2041 ctagagaatg agcaattaag gcggatagtg cctctgagac gctctaaaaa cggttttgaa
     2101 gcgagtttgg aacgagctaa agaaaaaagt aagggagaga gcatagagcg tcttaggaac
     2161 gaaaatagag cgcttagaag tgaaaactca gttttgagac agcaaaatga caaaatgcta
     2221 ggaaaactga aagagtttat gccagataaa gccttaaaaa attttatatc agagttaaga
     2281 gctattcagc caatcgtaag ggtagttaaa cgagtgattg aaaaagggct aggcctttga
     2341 gcgatttatg ccgtgaaagc taattgacaa taagcaaggg caaagtacgc taggacgtga
     2401 cgagccgaaa ggctttagcg tttcgagccg acacggacaa aggacgtccg cccttggtta
     2461 cttgttgtca attagaccat ggaataaagt aagcggacat ggtataatag ctaggtcgca
     2521 acgttctttc gctaagttac gaacttagat tggaggtgag cgctgtgaag actttcctag
     2581 aacttgtttt gatacctttt gtggttggcg ttgctgcaga ggtaagtgct gattggttga
     2641 ttcggtatgt tcgaaacaaa cgcgacaaaa attaacctga gttctttttg aggacaaaaa
     2701 agaaagacag tagttccagc tactgttttt tttgcgttgt gctattcgtt tcctagaact
     2761 tctagcgtta aaattattat accacgtttg gatttagaaa gtcaaaattt gaggttttag
     2821 gggtgaattt ttcgtgaaac gaaaaagagg gctgaaaagc ccttaaaaac ccatgcgtag
     2881 caagggtttt ttcttatctt gatacatata gaaataatga gtttttttat tttcttgttt
     2941 taaagcacct caaacccttg atattgctgg gtttttaggt aacaaaaaag cccttgcaat
     3001 ttaataaaat aaagtttata atttaagtgt ccaatttaaa ataataaact taggagaatt
     3061 gcaggaactt ttttatatac tcaaaaaaat ttttttgcaa gaaaattata acatgacagg
     3121 tactgaaaat caagtcttta aggactattc taagaatggc aaagatagaa agtggcgaga
     3181 acgcaagtta aaaaatattg agcttgctgg tcgtttagag cgtttaggat atcgttcgtt
     3241 tgaacgggtc tatcaatgtg ccgaagtgtt gaagtttatc gaacaacaag acggcacgaa
     3301 aaaactctat cagtcttatt tttgcaaaaa taagctctgc ccaatttgta actggagacg
     3361 gtcaatgaag tatgcttatc aagctgaatt agtggtaaat gaagcaatga aacgctatcc
     3421 aaaaggtcgc tttctctttt tgaccttgac gattaagaac attagtggtg aaaaattaaa
     3481 taaatcaatt tcagaaatag ggcgagcttt taatcgtctt atgaaataca agaaagtcga
     3541 taaaaatgtt attggctatt tgcgagccac tgaggtaact tattcaactg agcatgagaa
     3601 ttatcaccct catttgcatg tattgttatt tgtgaaatct agctatttta ctggaaataa
     3661 tacaaattac attagccaag aagaatggac gaaactatgg gctaaggcga tgaaattgga
     3721 ttatacacct gtagttgata ttcgaaccgt caaagctcat aaacgtaaaa acttgaagtc
     3781 agctattatc gaaacagcta aatatcctgt taagcctttt gatgtagata cagaagatgt
     3841 gacattattc tctgaaatgg tcaaagaacg gataacagaa gatttaacga atggtttgca
     3901 ccgaaaaagg cagattggtt ttggaaagtt gttcaagaaa atcaaggcgg agttagctct
     3961 tgatgatgtc gaagaaggga atcttgttca gaccggagca gaagaatctg cagaaagtac
     4021 tggtcgtgaa attgttgcct tttggaattg ggatagaaag aattattttg tgaggtagct
     4081 gatgagtaaa acagtatcag aattagctca agaattggga gttagtaggc agtatcttaa
     4141 tcggatttta tcgcaaaata atctcggtcg aaaaaaaggg aataaaaaag tagtttccga
     4201 tatggacgag aaagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4321 caacgatcgg aggaccgata caaattcctc gtaggcgctc gggaccccta tctagcgaac
     4381 ttttagaaaa gatataaaac atcagagtat ggacagttgc ggatgtactt cagaaaagat
     4441 tagatgtcta aaaagcttgt agttaaagct ttttagacat ctaaatctag gtactaaaac
     4501 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
     4561 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
     4621 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
     4681 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
     4741 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
     4801 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
     4861 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
     4921 tgaaagagcc tgatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4981 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5041 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5101 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5161 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5221 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5281 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5401 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5641 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5701 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5761 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
