LOCUS       sequence-146798-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pHAGE-TO-dCas9
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..85)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gattatctag attaccctac tcgaggctcc catcgatgcc agagcggccg ccagatccgg
       61 atcctacctt cctcttcttc ttgggagagc cactagtaga gccgtctcca ccgagctgag
      121 agaggtcgat tcttgtttca tagagccccg taattgactg atgaatcagt gtggcgtcca
      181 ggacctcctt tgtagaggtg taccgctttc tgtctatggt ggtgtcgaag tacttgaagg
      241 ctgcaggcgc gcccaagttg gtcagagtaa acaagtggat aatgttttct gcctgctccc
      301 tgatgggctt atccctgtgc ttattgtaag cagaaagcac cttatcgagg ttagcgtcgg
      361 cgaggatcac tcttttggag aactcgctta tttgctcgat gatctcatca aggtagtgtt
      421 tgtgttgttc cacgaacagc tgcttctgct cattatcttc gggagaccct ttgagctttt
      481 catagtggct ggccagatac aagaaattaa cgtatttaga gggcagtgcc agctcgttac
      541 ctttctgcag ctcgcccgca ctagcgagca ttcgtttccg gccgttttca agctcaaaga
      601 gagagtactt gggcagctta atgatgaggt cttttttgac ctctttatat cctttcgctt
      661 cgaga
//
